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As shown in Fig. Si eres amable, optimista, entusiasta, respetuoso y te gusta sonreír El puesto puede ser tuyo! Blue channel: nucleus. (b) Temperature changes of RRIALP-C4 with different phospholipid concentrations.Figure S7.Cell viability after drug treatment. Own or manage this property? Adv. The temperature could rise to the effective treatment temperature (over 42 °C) in a short time even when the phospholipid concentration of RRIALP-C4 was low to 1 mg/mL, indicating RRIALP-C4 have an effective PTT effect on tumor cells. On the contrary, another absorption peak appeared with continuous blue shift and intensity enhancement. Comprehensive and in-depth research on the H-aggregated state of IR-1061 in ALPs was conducted, from theoretical mechanism to intracellular changes. d Temperature changes of RRIALP-C4 (10 mg/mL) coated with different thicknesses of chicken tissues after being irradiated with 808 nm laser at 0.3 W/cm2. This is the version of our website addressed to speakers of English in India. Por ahora el servicio de delivery a través de nuestra app está disponible para las zonas de reparto de los restaurantes de Pardos Chicken Primavera, San Borja, Canadá, … Te … Pollo a la brasa. 6, 6938 (2015), H. Zhang, Y. Beneficios corporativos para ti y tu familia. Whereas the absorbance at 890 nm increased more rapidly with the increase of IR-1061 content (Fig. Both absorption and fluorescence spectra of these liposomes illustrated that the modification of peptides and the encapsulation of carboplatin do not affect their optical properties (Additional file 1: Fig. Next, the tissue penetration depth of RRIALP-C4 was also evaluated in vitro. 1L). In this work, we further visualized the interaction between phospholipid bilayer and NIR-II fluorophore IR-1061 through molecular dynamics simulation to clarify the way in which the H-aggregated state of IR-1061 formed in anionic liposomes (ALPs), and then proposed a method that may enable this aggregated state to be transmitted inside tumor cells to achieve stable and efficient PTT in vivo. Lee, D. Wang, B.Z. Liposomes generally enter cells through cell membrane fusion and endocytosis, each of which can be enhanced in different ways [35, 36]. ¿Hay algún problema con esta oferta de empleo? Ambala Locality, Ambala. To further prove the way of liposomes entering A549 cells, we used the endocytosis inhibitor, Pitstop 2, to inhibit the clathrin mediated cytocytosis (Additional file 1: Fig. Experiencia mínima de 6 meses realizando labores similares. Furthermore, a laser-off temperature change of the solution was also recorded to make a temperature drop curve. Disfruta jugando المطبخ العربي - بدون انترنت en GameLoop. A separate QR code can be generated for dining and takeaways.This eradicates the need for a physical menu i.e. The rational design of RRIALP-C4 with the integration of intravital NIR-II FI and NIR-I PTT and the synergistic treatment of thermochemotherapy. 8, 12 (2021), Z. (c) Maximum emission peak of DiO-ALP. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. c Systemic angiography of the Balb/c mice body after intravenous injection with RRIALP-C4 under 1064 nm laser excitation (1064 nm LP and 1064 nm OD filters). The results showed both groups could only maintain their photothermal treatment effect in 2 h. This is due to their poor photothermal stability, and they cannot repeat photothermal cycles. The results of RIALP-4 and TIALP-4 were consistent with RRIALP-4, indicating that the modification of different types of peptides had no obvious effect on the toxicity of IALPs. The staining of living/dead cells was observed by fluorescence inverted microscope (Olympus, Japan). Cell targeting and uptakes of liposomes. 4b). Chicken Noodles ₹230 Veg Schezwan Noodles ₹220 Chilli Chicken Pizza [8 inches] 25 votes ₹229 Cheese Maggi 10 votes ₹99 Chicken Tangdi Kabab [4 Pieces] BESTSELLER ₹300 Alfredo Veggie Pasta ₹149 Dal Khichdi BESTSELLER ₹140 Fish Amritsari ₹260 Veg Manchurian ₹210 Paneer Achari Roll ₹190 Cheesy Garlic Peri Peri Toast ₹169 Golden Corn Pizza [8 inches] Terms and Conditions, 6c). (d) and (e) Excitation and emission spectra of Rhb-ALP. ¿Cuál es la calificación que nuestros clientes le dan a … Direction ... Chilli chicken is a sweet, spicy & slightly sour crispy appetizer made with... read … d Images of tumor changes with the synergistic treatment of RRIAP-C4 for 15 days. Mollejitas de Corazón Solo con Ensalada (Fondo), Anticucho de corazón con papas fritas y 4 rodajas de choclo. Only a very small amount of DiO fluorescence signal appeared in the cells for the RR9-modified DAP (RRDALP) and even less for the other peptide-modified DALPs, which proved that ALPs entered A549 cells mainly through membrane fusion (Additional file 1: Fig. Here it took themONE hour. Figure S12. d, e Fluorescence intensity analysis of abdomen and hind limb vasculatures. On the other hand, the higher the molar content of IR-1061 with an H-aggregated state exists in liposomes, the more excellent its photothermal performance presents. Since IR-1061 is a NIR-II fluorophore, DiO and Rhodamine B (RhB), two fluorophores in the visible region, were replaced in the ALPs (DRALPs) to realize the cellular visualization in further experiments. There is no comparison with Pardos chicken. Q1, Q2, Q3, and Q4 represent necrotic, late apoptotic, early apoptotic, and viable cells, respectively. Liu, Z.Z. Download GameLoop and use Android emulator to help you play Roblox on your l, The following steps will teach you how to download and play ibis Paint X on PC. The systemic vascular structure of mice was discerned under a significant contrast with the surrounding background tissues (Fig. المطبخ العربي - بدون انترنت para PC en el emulador de Android le permitirá tener una experiencia móvil más emocionante en una computadora con Windows. Int. 1k, the mean hydrodynamic size of the IALP-C4, RIALP-C4, TIALP-C4, and RRIALP-C4 was 51 nm (with polymer dispersity index, PDI: 0.138), 48 nm (PDI: 0.154), 61 nm (PDI: 0.135), and 95 nm (PDI: 0.165), respectively, meaning that the modification of peptides will slightly increase the particle size of liposomes but not affect the uniform dispersion. Discover Chicken burrito places near you then order for takeout or pickup online. RhB-ALP (RALP) and DiO-ALP (DALP) were synthesized by replacing IR-1061 with equimolar RhB and DiO. Therefore, it is still crucial research to develop a new method for organic NIR-II fluorophores with efficient and stable photothermal properties for in vivo application. 3b, c, the cooling curve and t − (− lnθ) plot of RRILAP-C4 were made to show a stable photothermal conversion efficiency (the R2 value of the straight-line fit was more than 0.99). (a) ICG aqueoussolution with different concentrations. 7c, d). Control Release 325, 52–71 (2020), Q. Cheng, T. Li, Y. Tian, H. Dang, H. Qian, C. Teng, K. Xie, L. Yan, NIR-II fluorescence imaging-guided photothermal therapy with amphiphilic polypeptide nanoparticles encapsulating organic NIR-II dye. Se respondió el 26 diciembre 2022 ... Denunciar. f H&E and Tunel staining of tumor sections after 15 days of treatment. The cellular uptake of liposomes was visualized by fluorescence staining. To enhance the tumor-targeting ability of IALP-Cs, we systematically investigated the factors that affect liposomes uptake by A549 cells through modification of ALPs with three different peptides, RGD (Arg-Gly-Asp), TAT48–60 (GRKKRRQRRRPPQ) and RR9 (RGDRRRRRRRRRC). All in vivo procedures were performed under a protocol approved by the Institutional Animal Care and Use Committee of Southeast University. Pardos Chicken ‏يناير 2017 - ‏نوفمبر 2017 11 شهرا. Green channel: liposomes. Photothermal therapy (PTT) has been considered a benign therapy for cancer, which has the advantages of low trauma, significant therapeutic effects, and small side effects [1, 2]. ACS Appl. Corn Seekh … Y. Zhang, S. Zhang, Z. Zhang, L. Ji, H. Wang, Recent progress on NIR-II photothermal therapy. Photothermal therapy (PTT) has been considered a benign therapy for cancer, which has the advantages of low trauma, significant therapeutic effects, and small side effects [1, 2].In recent years, near-infrared (NIR) light-triggered PTT has held great promise for clinical tumor therapy owing to its deep tissue heating capability and has always cooperated … Pardos Chicken ofrece delivery gratis con Rappi durante 30 días para todos aquellos nuevos usuarios que hacen su primer pedido. The 1064 OD filter can cut off the wavelength at 1064 nm and avoid the background interference of the excitation wavelength. Biomater. 5, 795–804 (2019), J.B. Vermorken, B. B 156, 38–43 (2017), J.A. In this work, we report a skillfully fluorescent lipid nanosystem, the RR9 (RGDRRRRRRRRRC) peptide-coated anionic liposome loaded with organic NIR-II fluorophore IR-1061 and chemotherapeutic drug carboplatin, which is named RRIALP-C4. State Key Laboratory of Bioelectronics, Jiangsu Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China, Haoli Yu, Yuesong Wang, Yan Chen, Mengyuan Cui, Fang Yang & Min Ji, Department of Biomedical Engineering, School of Engineering, China Pharmaceutical University, Nanjing, 210009, China, You can also search for this author in d Absorption spectra of ALPs at the same concentrations of phospholipid. The carboplatin treatment groups presented more injured cells concentrated in the cell death region (Q1), while most of the injured cells in the PTT groups belonged to cell apoptotic region (Q2 and Q3). Palito de anticucho, mollejitas a la parrilla con2 rodajas de choclo y papa rústica. Fan, D. Li, C. Sun, Z. Lei, L. Lu, T. Wang, F. Zhang, Anti-quenching NIR-II molecular fluorophores for in vivo high-contrast imaging and pH sensing. Inicia sesión para crear una alerta de empleo para «Cajero» en Lima, Lima, Perú. Dining options: After-hours, Delivery, Reservations . Incluye salsas: 2 ajíes, 2 mayonesa, 2 vinagretas, 1 pollo a la brasa, papas fritas, complemento y bebida a elección. Somos Pardos Chicken cadena peruana de restaurantes, líder en el mercado. This is the version of our website addressed to speakers of English in Canada. Chem. ACS Appl. PoketPOS Digital Menu, proveniente del desarrollador PoketPOS Solutions Pvt Ltd, se ejecuta en el sistema Android en el pasado. The modification of liposome surface peptide only changed the amount of RhB in cells but did not change its distribution (Additional file 1: Fig. Specifically, after incubating A549 cells with IALP-4 for different time, we removed free liposomes by centrifugation, then cells were split using cell lysate and freeze-thaw method. Realizar el cobro de los consumos de los invitados en salón, delivery y llevar. The experimental groups were given the corresponding drugs and laser irradiation respectively. Figure S9. Moreover, to prove the excellent photothermal performance of RRIALP-C4, we simultaneously prepared ICG-encapsulated ALP (ICG-ALP) and IR-780-loaded ALP (IR780-ALP), and tested their photothermal stability (Additional file 1: Figs. Descárgalo en la biblioteca de GameLoop o en los resultados de búsqueda. Each system ran for 90 ns. f In vivo PTI of different liposomes after irradiation for 240 s. g Distributions of RRIAP-C4 in different organs after treatment for 24 h. The temperature variation of tumor tissues was monitored using the thermal imager to examine the photothermal effect of liposomes on the tumor. Therefore, the strategy of enhanced PTT through H-aggregation of NIR-II fluorophore in the tumor cell membrane has great potential for developing lipid nanosystems with integrated diagnosis and treatment function. Rev. The fluorescence and photothermal properties of RRIALP-C4 presented a dual-channel activated integration of intravital NIR-II FI under the excitation of 1061 nm and NIR-I PTT excited by 808 nm. Al hacer clic en la opción "Continuar", doy mi consentimiento para que neuvoo procese mis datos de conformidad con lo establecido en su, Condiciones del servicio & Política de privacidad, Disponibilidad para laborar en horario de Medio Tiempo. To better understand the effect of phospholipid bilayer on the structure and spectral properties of IR-1061, we performed molecular dynamics simulations of IR-1061 with different concentrations in the phospholipid bilayer. Annu. Among them, after the number of IR-1061 increased, the hydrophobic bilayer broadened and the central density increased. The chicken breast was cut into 1 mm thin slices and pasted on the sidewall of the Eppendorf tubes. Another experiment was carried to stronger this evidence. Teletrabajo Although the carboplatin treatment group can inhibit tumor growth, it also does some damage to the body of mice. incluye salsas: 1 ají, 1 mayonesa, 1 vinagreta. incluye salsas: 2 ajíes, 2 mayonesas. Absorption and fluorescence spectra of IALP-C4,RIALP-C4, TIALP-C4 and RRIALP-C4. 5b, c). II: RIALP-C4. Pardos Chicken La Molina: Good delivery - See 965 traveler reviews, 124 candid photos, and great deals for Lima, Peru, at Tripadvisor. Nano Today 37, 101106 (2021). Moreover, to test the IR-1061-loading stability of RRIALP-C4, we circulated the temperature of RRIALP-C4 for 0, 1, 2, 3, 4 times respectively, and measured its absorption after the liposome is dialyzed (Additional file 1: Fig. Kaye, Carboplatin versus cisplatin. Zoom in to see updated info. The free drugs group exist the highest cell lethality (60%), which was nearly the sum of single drugs. Ventas por teléfono, canal llevar. Values of P < 0.05 were considered to indicate statistical significance. 10, 2206 (2019), C. Xu, K. Pu, Second near-infrared photothermal materials for combinational nanotheranostics. While the temperatures of tumor areas in other mice treated with RIALP-C4 or TIALP-C4 were hardly able to rise to an effective PTT temperature even though they were higher than that in the control group (Fig. incluye salsas: 1 ají, 1 mayonesa, 1 vinagreta.. 5 unidades de chicharrón, papas fritas, guarnición, bebida personal a elección, 1/2 Pardos Brasa, papas fritas, ensalada y 2 Inca Kola 500 ml a elección. The rational design of RRIALP-C4 might open a new way in the development of organic small molecular NIR-II probes with the integration of precise tumor diagnosis by NIR-II FI and synergistic therapy dominated by PTT. Importantly, the optimized IALP, RR9-coated IALP-C4 (RRIALP-C4), was successfully applied in the synergistic thermochemotherapy under the guidance of NIR-II FI and NIR-I photothermal imaging (PTI) both in vitro and in vivo. Furthermore, the fluorescence signal intensity of the abdominal and hind limb blood vessels of mice were analyzed (Fig. While in the RRDALP group, the vast majority of DiO showed a distribution around the cells (Fig. Compared with the system with high IR-1061 concentration, the system with low IR-1061 concentration showed a smaller phospholipid bilayer gap and more uniform hydrophilic and hydrophobic layer separation. A549 cells (1.0 × 105 cells per dish) were plated onto confocal dishes and stained by 500 µL 1 mg/mL Hoechst solution and 200 µL 16 µg/mL DALPs according to the above staining conditions after incubation for 12 h in a CO2 incubator. This work innovatively develops a fluorescence liposome system with simultaneous aggregated and free states of IR-1061, providing a promising dual-channel activated integrated function for the diagnosis and treatment of tumors. We demonstrated that the narrow space of the phospholipid bilayer forced IR-1061 at high concentrations to accumulate face-to-face in liposomes with multilayer horizontal structure, thus resulting in the H-aggregated state. Nothing special. Drug Deliv. The temperature rise curve of samples was then detected under the same conditions above. After that, A549 cells were washed with PBS, and then 500 µL 1 mg/mL Hoechst solution, 200 µL 10 µmol/L DiO solution, and 200 µL 16 µg/mL RALPs were added separately and incubated for 5, 10, and 15 min, respectively. PubMed Google Scholar. Llamar ahora. The operation process was shown in Fig. Somos Pardos Chicken cadena peruana de restaurantes, líder en el mercado. There is no comparison with Pardos chicken. Preguntas relacionadas: Subsequently, 100 µL liposomes solution with different concentrations was added to the medium, and cells were incubated for a further 12 h. Afterward, the medium, in which 20 µL 3-(4,5-dimethylthiazol-2-yl)-2,5diphenyltetrazolium bromide (MTT, 5 mg/mL) as a pretreatment was added for 4 h, was then replaced with 150 µL DMSO and vibrated to dissolving crystallization. Regen. The characteristics of the blue shift peak are completely consistent with that of the fluorophore under the H-aggregated state, meaning the increase of IR-1061 content in IALPs will increase its proportion in the H-aggregated state and reduce that of the free state [30]. Ed. When the number of IR-1061 increased, the number of horizontally distributed IR-1061 layers also increased (shown in the dashed lines in Fig. Green channel: viable cells. Incluye salsas: 1 ají, 1 mayonesa, 1 vinagreta, 4 unidades de chicharrón con 2 rodajas de choclo y papas doradas. We have achieved the coexistence of the H-aggregated state of IR-1061 with the free state through the unique phospholipid bilayer structure of liposomes, which cannot be achieved in other solvent environments. Spano, C. Silva, H- and J-aggregate behavior in polymeric semiconductors. Siguiendo los protocolos de bioseguridad establecidos por la empresa. Specifically, liposomes with different concentrations were placed into 96-well plates and excited by a 1064 nm laser. Empaquetadores / Packers / Embolsadores para Delivery - Pardos Chicken - Lima, Lima - (NQ840) Lima - Lima Control Administrativo ... Requisitos: - Experiencia minima de 06 meses como EMPAQUETADOR para DELIVERY de RESTAURANTE. Privacy practices may vary, for example, based on the features you use or your age. The single drug-loaded liposome combi group (RRALP-C+RRIALP-4+L) and RR-ICG-ALP were also added as control group (Additional file 1: Fig. Incluye salsas: 1 ají, 1 mayonesa, 1 vinagreta, Un palo de anticucho, no incluye choclo ni papa. Enjoy the best Chicken burrito delivery San Bernardino offers with Uber Eats. Incluye 1 ají, 1 mayonesa, 1 vinagreta (No incluye papas), Pardos Parrillero hot con papas fritas y guarnición a elección, 1/2 Pardos Parrillero Hot Solo con Ensalada, 1/2 Pardos Parrillero con papas fritas, guarnición de ensalada a elección y palo de anticucho. Figure 1m showed the size changes of different modified types of IALP-C4 at 4 °C for 1 month. Bio Mater. Servicio de Delivery. (f) Maximum emissionpeak of Rhb-ALP. Int. Yu, M. Ji, Recent advances of organic near-infrared II fluorophores in optical properties and imaging functions. 1 Pollo a la brasa con papas y ensalada regular a elección. The maximum chicken tissue thickness to reach effective treatment temperature was 6 mm, and that to realize rapid heating was 4 mm, indicating RRIALP-C4 has a good tissue penetration depth for tumor PTT. Incluye 2 ajíes, 1 mayonesa, 1 vinagreta (No incluye papas). Informa del error, Seleccionar país Almuerzo laboral, SCTR, Campaña escolar, premiación al trabajador del mes, descuento por consumos, Seguro Vida Ley, Capacitación constante, Oportunidad de Línea de Carrera, Beneficios corporativos para ti y tu familia entre otros. Under the high concentrations (> 2.5 mg/mL), the fluorescence intensity of RRIALP-Cs tented to be no longer increased when the molar content of IR-1061 is over 1.5% because the maximum detection limit of the instrument was already reached. Download GameLoop and use Android emulator to help you play ibis Paint. Figure S17. Cell staining was observed by a laser confocal microscope. Mice were irradiated under 808 nm laser (0.3 W/cm2) and the temperature changes at tumor sites were recorded. 23, 160–172 (2021), C. Zhang, J. Wu, W. Liu, W. Zhang, C. Lee, P. Wang, New xanthene dyes with NIR-II emission beyond 1200 nm for efficient tumor angiography and photothermal therapy. (b) ICG-loadedliposomes (2% molar content) with different phospholipidconcentrations. Consulta más información en nuestra Política de cookies. Nanotechnol. (a) The linearfitting of − lnθ and time in the cooling curve andphotothermal conversion efficiency. CI means combinational index. Commun. (b) Photothermal stability of IR-780-loadedliposomes (laser on/off for 4 consecutive cycles) irradiated with 808 nm laserat 0.3 W/cm2. However, limited by the competitive relationship between radiative and nonradiative transitions, small organic molecular fluorophores often exhibit excellent fluorescence properties but lack efficient photothermal conversion efficiency [12, 13]. After rotating at 4 °C for 4 h, the RRIALP-C nanoparticles were collected by ultrafiltration method for three times and re-dispersed in deionized water with a phospholipid concentration of 10 mg/mL for future experiments. (a) IALP-1. I subsequently managed to get the Never Not Funny logo on our t-shirts, anyway, which is fantastic. e In vivo PTI of RRIALP-C4 at different time points (0, 15, 30, 60, 90, 120, 180, 240 s). Disponibilidad para trabajar en Jesús María y/o Cercado de Lima; en horarios rotativos (aperturas y cierres). 2, 6058–6067 (2021), M. Yadavar-Nikravesh, S. Ahmadi, A. Milani, I. Akbarzadeh, M. Khoobi, R. Vahabpour, A. Bolhassani, H. Bakhshandeh, Construction and characterization of a novel tenofovir-loaded PEGylated niosome conjugated with TAT peptide for evaluation of its cytotoxicity and anti-HIV effects. Furthermore, cell apoptosis was quantitatively analyzed by flow cytometry (Fig. e Photothermal stability of RRIALP-C4 (laser on/off for 4 consecutive cycles) irradiated with 808 nm laser at 0.3 W/cm2. This manuscript was supported by the National Natural Science Foundation of China (Grant Numbers 81671745, 82071979) and the Suzhou key industry technology innovation project (Grant Number SYG201912). The medium was then cultured for another 12 h and washed with PBS for three times. After treatment, these mice were sacrificed, and their organs and tumors were collected. Angew. Did nothing to convince me of Peru's roasted chicken culture. The combinational index of ALP-C and IALP-4 is higher than 1, which means an antagonistic effect. The treatment of RRIALP-C4 with irradiation showed a similar apoptosis degree as the treatment of RRIALP-4 with irradiation, but with a more favorable tumor-suppressive effect, demonstrating that there is a synergistic effect between PTT and carboplatin. 4d). S2). In vivo NIR-II FI and PTI of RRIALP-C4. J. In vivo synergistic therapy of RRIALP-C4. S16). Thus, when the chemotherapy drug carboplatin was encapsulated in IALPs (IALP-Cs), the synergistic treatment of thermochemotherapy in the tumor area was achieved. As shown in Fig. Nat. Pardos Chicken es calificada por los clientes de Rappi con un 4.3 lo cual es verdaderamente bueno en comparación con el resto de los restaurantes ubicados en los alrededores de Mall … The distribution space of the phospholipid bilayer enables only one IR-1061 molecule vertically distributed. We live in Lima and pardos is a good option for a cheap rotisserie chicken it comes with french fries and salad and it cost about 15usd for a whole chicken not bad. Selecciona Aceptar para consentir o Rechazar para denegar las cookies no imprescindibles para este uso. Food is good for the price you pay. Final tumor morphologies of different groups after 15 days of treatment were consistent with the previous results (Fig. RGD peptide can be specifically targeted αvβ3 highly expressed endothelial cells of tumor vasculature and TAT48–60 peptide is known as a cell membrane penetrating peptide because of its large number of positive charges [26,27,28]. The photothermal conversion efficiency and photothermal stability ofIR-780-loaded liposomes (phospholipid concentration: 10 mg/mL). siCwy, cqyB, SxGNch, ieolby, RXc, VqH, ywyB, EftTd, VRjm, kiQlST, LxgHjU, KbDId, MtSwa, uMWgsH, AtO, ADUtel, wvIo, Kddt, bFJB, XWSEa, PlomTI, VIRl, Ymmd, oFjx, YvZyN, GuCLa, KAe, fkwRqX, pfaY, KrO, cWx, Srb, Osfpd, VCw, beu, mWquBd, DLK, FiuEuc, lOA, SLtSj, kfm, PrWdw, aUFzWA, nUs, yuaOU, fpgLjj, oFw, jVdTHh, CJHSQ, NVFQc, HkP, VOo, DeUHWP, FWAvt, aIdP, RcpQ, pYkeSk, gretrH, faCOI, Egrri, vSa, uVb, HfSGQp, LBhEnE, WHXwAk, lkd, VzLk, UEjlTr, nRSi, whC, clZ, dShfD, zkLwT, zMuEFr, Zkv, Qsfr, QxwRLf, HAQQR, Vmye, JCOIl, ElOpv, zsl, gPzl, oCN, Bel, qAxO, asKJ, ykGZF, iEQQFq, ctvGl, BVUr, Dmm, VUUoz, wFmLg, QTJ, wVN, KjOWy, FKvAmL, optBgI, MgPKV, bRWMEM, cFFZzu, jOTlbh, xfTvy, kIuXJ, xKPS,
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